RD 114 Virus-Specific Sequences in Feline Cellular RNA: Detection and Characterization
نویسندگان
چکیده
منابع مشابه
Feline leukemia and RD-114 virus group-specific proteins: comparison of amino terminal sequence.
The major internal virion polypeptide from feline and RD-114 type C viruses has been subjected to amino terminal sequence analyses with the Beckman automated sequencer. These proteins, as well as their homologs in rat and mouse viruses, begin with the sequence prolylleucylarginyl (Pro-Leu-Arg). Virus RD-114 differs from conventional feline type C viruses that show about 80 percent relatedness b...
متن کاملStructure, subunit composition, and molecular weight of RD-114 RNA.
The properties and subunit composition of the RNA extracted from RD-114 virions have been studied. The RNA extracted from the virion has a sedimentation coefficient of 52S in a nondenaturing aqueous electrolyte. The estimated molecular weight by sedimentation in nondenaturing and weakly denaturing media is in the range 5.7 X 10(6) to 7.0 X 10(6). By electron microscopy, under moderately denatur...
متن کاملCharacterization of enhancer elements and their mutations in the long terminal repeat of feline endogenous RD-114 proviruses.
To locate the enhancer regions of the feline endogenous RD-114 long terminal repeat (LTR), we examined expression of the chloramphenicol acetyltransferase gene driven by various segments of the U3 region from two different proviral loci (CRL3 and CR1). Transient expression assays demonstrated that the primary signal sequence for transcription enhancement was located within the 63-base-pair (bp)...
متن کاملPurification and immunological characterization of the major internal protein of the RD-114 virus.
The major internal protein of the RD-114 virus that appeared in a human tumor cell line, RD, after passage through fetal cats was purified by isoelectric focusing and used to prepare antibody in guinea pigs. The protein has a molecular weight of 33,500 in sodium dodecyl sulfate-polyacrylamide gels, and an isoelectric point of 9.1. This contrasts with a molecular weight of 25,000 and an isoelect...
متن کاملEndogenous feline retrovirus RD-114 does not elicit neutralizing antibodies in dogs.
At least two retroviruses, endogenous feline leukemia virus (FeLV) and RD-114 virus, are assumed to be integrated in the genome of domestic cats (1). Several feline cell lines have been shown to produce RD-114-related viruses (2). In Japan, feline cell lines are widely used to produce vaccines for dogs, particularly against canine parvovirus. The level of infectious RD-114 virus found in canine...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1973
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.12.5.984-994.1973